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esrrg orf  (Millipore)


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    Millipore esrrg orf
    Esrrg Orf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/esrrg+orf/esrrg+orf/ppr0783306-181-21-29
    Average 90 stars, based on 1 article reviews
    esrrg orf - by Bioz Stars, 2026-09
    90/100 stars

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    Expressing:

    Article Title: Loss of ERRγ promotes lineage plasticity towards neuroendocrine prostate cancer
    Article Snippet: .. Viral infections 388 consisted of co-transfection of lentivirus packaging vector psPAX2 (Addgene, Cat#12260) and 389 envelop vector pMD2.G (Addgene, Cat#12259) with ESRRG ORF (McGill expression library, 390 Cat#TRCN0000489295 from Sigma) into HEK293T cells to first generate lentivirus. .. DU145 cells 391 were infected by the virus supplemented with 8 μg/ml polybrene (Sigma, Cat#H9268) and selected 392 by 1 μg/ml puromycin (Sigma, Cat#P8833).



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    Figure 2: ERRβ/γ agonist DY131 is growth-inhibitory in breast cancer cells. A. Crystal violet staining of breast cancer and non-transformed breast epithelial cell lines in the presence or absence of the indicated concentrations of DY131 or DMSO control over time. N = 6 for a representative assay performed in sextuplicate, two-way ANOVA with Bonferroni post-tests in each cell line vs. DMSO control at Day 10/11. B. Clonogenic survival assay for MCF7 and MDA-MB-231 cells seeded at low density and cultured for 13 d after a single, 24 h exposure to the indicated concentrations of DY131. C-D. Representative Western blot analysis of basal ERRβsf, ERRβ2, and <t>ERRγ</t> expression in non-transformed mammary epithelial and breast cancer cells. β2 and βsf positive controls are from MDA-MB-231 cells transiently transfected with the indicated cDNA. γ positive control <t>is</t> <t>purified</t> protein.
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    Figure 2: ERRβ/γ agonist DY131 is growth-inhibitory in breast cancer cells. A. Crystal violet staining of breast cancer and non-transformed breast epithelial cell lines in the presence or absence of the indicated concentrations of DY131 or DMSO control over time. N = 6 for a representative assay performed in sextuplicate, two-way ANOVA with Bonferroni post-tests in each cell line vs. DMSO control at Day 10/11. B. Clonogenic survival assay for MCF7 and MDA-MB-231 cells seeded at low density and cultured for 13 d after a single, 24 h exposure to the indicated concentrations of DY131. C-D. Representative Western blot analysis of basal ERRβsf, ERRβ2, and ERRγ expression in non-transformed mammary epithelial and breast cancer cells. β2 and βsf positive controls are from MDA-MB-231 cells transiently transfected with the indicated cDNA. γ positive control is purified protein.

    Journal: Oncotarget

    Article Title: Antimitotic activity of DY131 and the estrogen-related receptor beta 2 (ERRβ2) splice variant in breast cancer.

    doi: 10.18632/oncotarget.9719

    Figure Lengend Snippet: Figure 2: ERRβ/γ agonist DY131 is growth-inhibitory in breast cancer cells. A. Crystal violet staining of breast cancer and non-transformed breast epithelial cell lines in the presence or absence of the indicated concentrations of DY131 or DMSO control over time. N = 6 for a representative assay performed in sextuplicate, two-way ANOVA with Bonferroni post-tests in each cell line vs. DMSO control at Day 10/11. B. Clonogenic survival assay for MCF7 and MDA-MB-231 cells seeded at low density and cultured for 13 d after a single, 24 h exposure to the indicated concentrations of DY131. C-D. Representative Western blot analysis of basal ERRβsf, ERRβ2, and ERRγ expression in non-transformed mammary epithelial and breast cancer cells. β2 and βsf positive controls are from MDA-MB-231 cells transiently transfected with the indicated cDNA. γ positive control is purified protein.

    Article Snippet: Human ERRγ purified protein (transcript variant 2), was purchased from Origene (Rockville, MD).

    Techniques: Staining, Transformation Assay, Control, Clonogenic Cell Survival Assay, Cell Culture, Western Blot, Expressing, Transfection, Positive Control, Purification